Effect of growth regulators on callus induction in Rice embryo culture

نویسندگان

  • P. Shanthi
  • S. Jebaraj
  • S. Geetha
چکیده

An experiment was conducted to study the effect of growth regulators on callus induction in rice embryo culture. In this experiment, caullogenesis was initiated from the matured seeds of seven indica rice varieties viz., TRY 1, TRY 2, Pokkali, CSR 10, W.Ponni, BPT 5204 and IR 29. The medium used for this callus induction was Murashige and Skoog (1962) commonly referred as MS medium with six different combinations of growth regulators viz., MS+2,4-D 25mMl, MS+2,4D 20mMl, MS+2,4-D 15mMl, MS+2,4-D 10mMl MS+2,4D 15mMl+ KIN 2.5mMl, MS+2,4-D 10mMl+ KIN 2.5 mMl Among the above combination of the growth regulators, MS + 2 mMl 2,4,D + 0.5 Kinetin performed well irrespective of all the genotypes. Analysis of variation showed that the genotype and medium are significantly different from each other and the interaction between Genotype X Medium also highly significant. Among the seven genotypes, Pokkali was significantly at (1%) superior for callus induction (63.57%) followed by W.Ponni (53.96%). The genotypes TRY 2 (51.70 %), TRY 1 (50.87) and CSR10 (49.22) are significantly at (5%) on par with each other. The well developed callus was transferred to regeneration medium for regeneration. The medium combination of MS +Kin 5mMl + BAP 5mMl + NAA 0.5mMl recorded maximum average regeneration frequency of 57.25 percentage and higher response than the others. Among the varieties, Pokkali showed maximum regeneration frequency of 39.41 per cent and CSR 10 recorded minimum regeneration frequency of 20.23 per cent.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Optimization of callus induction of Zataria multiflora under the effect of different plant growth regulators and explant source

Background and objectives: The Lamiaceae family is rich in favorable secondary metabolites which have different medicinal properties and also use in food, cosmetic and sanitary industry. Zataria multiflora Boiss. is an aromatic and bushy plant containing specific pharmaceutical components which is only distributed in certain regions of Iran. Tissue culture tec...

متن کامل

Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Elahe Baharan1, Payam Pour Mohammadi1, Ehsan Shahbazi2 and Seyedeh Zahra Hosseini3

The present study was carried out to evaluate the effects of light and plant growth regulators on callus induction and explant browning in date palm cultivar, Estameran in in vitro condition. The explants were pretreated by an antioxidant combination (100 mgl-1 citric acid and 150 mgl-1 ascorbic acid) for 3 days in 5 °C. Then, the  leaf explants  were cultured in Murashige and Skoog medium (MS)...

متن کامل

Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Milk Thistle (Silybum marianum L. Gaertn.) under Tissue Culture Medium

The Silybum marianum (L.) Gaertn. is the dicotyledonous herbs of the Asteraceae family that is important in medical industry. The biological active compound of S. marianum is a mixture of several flavonolignals generally known as silymarin. The purpose of this study was to optimize S. marianum tissue culture for callus induction, silymarin production and comparison of some biochemical traits be...

متن کامل

Effects of Plant Growth Regulators and Explant on Callus Induction in Cuminum cymium L.

Cumin (Cuminum cyminum L.) as a member of the Apiaceae family is one of the most important medicinal plants in Iran. The purpose of this study is to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. For this purpose the cumin seeds (Kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on MS basal medium. Leaf and hypo...

متن کامل

Tissue Culture and Production of Volatile Secondary Metabolites from Callus of Stevia rebaudiana at Different Combination of Plant Growth Regulators

 Stevia rebaudiana Bert (Asteraceae) is herbaceous plant with sweet leaves, which are the source of sweetener products. The present study focuses on effects of different combination of plant growth regulators on callus induction, callus cell growth and regeneration and volatile constituents from callus of stevia in tissue culture condition. The results of present study showed callus formation p...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2010